Lately I have been spending a lot of time and energy trying to run some HPLC analysis. I am working on my professor newest pet project, namely the umani taste. An important part of this is glutamate, so right now I am trying to measure that in various food material. My first attempt to do that was to use a kit from Sigma Aldrich, but that was a pain. For every sample I had to measure I had to do 11 pippeting steps (!) and marking 4 Eppendorfs. So with 10 standards and 16 samples It was a lot of work. And in all three attempts I never got a nice standard curve. That is usually the bad things with kit’s. They looks so simple and easy, but it always ends up in lot of work and bad results. At least in my hands. Therefore I needed a better and faster method and the choice here was HPLC, mostly because I could get access to a nice machine. Now I just need to find the right method, and I think I will try this one: http://www.akademiai.com/content/k462w785x4564162/
[...] there is my HPLC project, were I am trying to measure glutamate contend of some food stuff and related materials. [...]